现在使用色谱柱的方式正确吗？

  液相色谱柱在使用过程中属于消耗品,***后终将会被更换。在使用的过程中，你需要注意一些参数来观察色谱柱何时需要更换。比如：柱压的升高、拖尾情况、理论塔板数是否降低、保留位移……

  随着时间的推移，色谱柱分离效果变差的原因有很多。其中，你使用的样品对色谱柱后期的使用寿命有至关重要的作用。如果你使用的样品比较“脏”。它将会导致固体颗粒杂质滞留在筛板上，色谱柱的筛板和柱头发生堵塞。解决这类问题，我们强烈推荐你使用保护住或者过滤样品。如果已经发生了柱头或者筛板堵塞的情况，可尝试冲洗色谱柱或者反相冲洗色谱柱。

   当然，温度、反压、pH都会对柱子产生影响。所以在使用色谱柱之前，请仔细阅读色谱柱使用说明书。******在合理的范围内使用。

   同样重要的是，有时候也不总是色谱柱的问题影响结果的一致性，仪器的维护和清洗也十分的重要。当您试图用HPLC仪器解决问题时，保存一个良好的仪器使用日志将节省您的时间和******。

英文版

It is important to remember that HPLC columns are consumables. Eventually the column you are using will have to be replaced.there are several parameters that you should keep note of in order to know when your column should be changed.

Some of the items i keep track of are……

    pressure increases

    tailing peaks

    plate loss

    retention shifts

There are several reasons that a column will go bad over time. The samples you are using will play a big role . If using

" dirty " samples,a guard column is usually recommended. Samples can contain impurities that are absorbed onto the surface of the bonded phase or particles that could plug the frist of the column. In order to avoid these problems,flushing the column with a strong solvent(rinsing with water frist if using buffers)can help the column last longer or filter the samples. Storing the column in a strong solvent for long periods of time will also help. If problems still occur,putting the column on in the reverse direction could remove any potential blockage of the frits.

High temperatures, backpressures, and pH could also lead to column failure.Be sure to check the care and use sheets in your column box before running.

It is also important to note the column is not always the problem. Perisdic maintenance and cleaning of your instrument is very important in order to keep results consistent. Keeping a well-kept log book will save you time and money when trying to troubleshoot a problrm with your HPLC instrument.

                    更多技术支持，或者需要购买色谱柱，实验室仪器，耗材、欢迎拨打028-85115092或者搜索www.cdmole.com